High frequencies of antigen-specific memory CD8 T cells in SMG. (A) Percentages of CD8 memory T cells specific for LCMV gp33 peptide in spleen (SP), SMG, and IEL of LCMV-immune (4–16 wk p.i.) B6 mice determined by MHC class I tetramer (gp33/H-2Db) staining. (B) Percentages of CD8 memory T cells specific for VSV np52 in spleen (SP), SMG, and IEL of VSV-immune B6 mice determined by MHC class I tetramer (np52/H-2Kb) staining 28 wk p.i. (C) Percentages of CD8 memory T cells carrying the P14 TCR (Thy1.1) in spleen (SP), SMG, and IEL determined in LCMV-immune (4–16 wk p.i.) P14 chimeric mice. Representative dot plots and statistical graphs are shown. All samples were gated on CD45 cells. Percentages of CD8 T cells specific for the indicated markers are depicted. Dots in statistical graphs represent values from individual mice. **P < 0.01; ***P < 0.001.