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. 2011 Sep 15;108(40):16789–16794. doi: 10.1073/pnas.1113486108

Fig. 1.

Fig. 1.

Overexpression of REST during neurogenesis blocks migration. (A) Immunostaining of representative coronal brain sections, 2 d postelectroporation (E14–E16). (Upper) The migration of the electroporated GFP cells. ToPro3 (blue) represents nuclear staining and serves as a marker for the different cortical layers. (Lower) The coexpression of REST or RESTΔNΔC with GFP. The REST p73 antibody was used to detect both full-length REST and RESTΔNΔC (red). [Scale bars, 200 μm (Upper); 50 μm (Lower).] (B) Bar graphs represent the maximum distance in which GFP-expressing cells migrated into the IZ. The length of the IZ (blue bar graph) is taken as 100%; the maximum distance in which GFP-expressing cells (green) migrated (green bar graph) presented as a fraction of the total length of IZ (blue). Error bars represent SD based on an n value of at least 4 and three sections from each brain.