Fig. 6.

Overexpression of DCX partially rescues the migration and neuronal differentiation defects caused by sustained REST expression. (A) Representative images of immunostained brain sections. Brains were electroporated at E14, either with REST-IRES-GFP alone or with the DCX-expression vector, and harvested at E19 (n = 5). Smaller panels on the Right are enlargements of the framed areas in the Center. (Top) Migration of the electroporated GFP cells. DAPI (blue) represents nuclear staining and serves as a marker for the different cortical layers (fragmented red lines). Red arrows show radial migration. (Middle) Coexpression of DCX with GFP. Note the bipolar radially migrating cell on the Right. (Bottom) Coexpression of NeuN with GFP. (Scale bars, all 50 μm, except Top, which are 200 μm.) (B) ChIP analysis on NS/P cells indicating binding of REST to the RE1 site in the DCX gene. Coding region of the DCX gene serves as a negative control.