Fig. 5.

Clocked production of a MG aptamer. (A): Operation scheme of SWMG. SWMG is operated in parallel to switch SW21, but is used to produce a functional RNA molecule—the MG aptamer rMG—instead of regulatory RNA species rI2. When MG is bound to the aptamer, it becomes highly fluorescent. (B): The MG aptamer is not degraded by RNase H, and hence accumulates over time. The MG fluorescence signal grows whenever SW21 is on. Shown is the concentration of T21 (dark red) and MG aptamer (dark green) as well as the derivative of the MG signal at 100 nM SWMG concentration. (C): Oscillator time traces in the presence of 0 nM (black), 100 nM (dark red), 200 nM (red), 400 nM (orange) SWMG. (D): Corresponding fluorescence signals (converted to aptamer concentration) recorded from the MG aptamer.