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13C NMR spectrum of the purified ARS, 3,7,11-trimethyl-2,6,10-dodecatrienoate (or farnesoic acid), in CDCl3. 1H NMR (500 MHz, CDCl3) δ : 1.60 (s, 6, C-7 CH3 and C-11 CH3), 1.68 (s, 3, C-11 CH3), 1.98 (m, 2, H-9), 2.05 (m, 2, H-8), 2.18 (d, 3, J = 1.5 Hz, C-3 CH3), 2.20 (s, 4, H-4 and H-5), 5.08 (m, 2, H-6 and H-10), and 5.70 ppm (s, 1, H-2). MS m/z (rel. int) 236 (M+ 0.8%), 193 (23), 136 (40), 121 (43), 100 (72), 81 (75), and 69 (100).
