Table 2.
Identified proteins of mare follicular fluid after depletion with MARS-6 and separation by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)
| Gene | Accession N° 1 | Protein name | Score2 | Theoretical | Peptide N°3 | Sequence coverage (%)4 | |
|---|---|---|---|---|---|---|---|
| pI3 | Mr (Da)3 | ||||||
| A1AT | gi|194225326 | Alpha-1-antitrypsin | 398 | 5.31 | 46896 | 6 | 13 |
| A1BG | gi|194216172 | Alpha-1B-glycoprotein | 374 | 8.74 | 68222 | 8 | 21 |
| A2M | gi|194211675 | Alpha-2-macroglobulin | 1406 | 6.24 | 163911 | 25 | 26 |
| AGT | gi|194206059 | Angiotensinogen | 50 | 7.06 | 70056 | 1 | 3 |
| ALB | gi|76363596 | Serum albumin precursor | 112 | 5.89 | 68494 | 2 | 4 |
| APOH | gi|149723623 | Apolipoprotein H | 33 | 8.43 | 38511 | 1 | 2 |
| CFB | gi|149732066 | B-factor, properdin | 139 | 6.75 | 85930 | 2 | 4 |
| DNAH7 | gi|149699076 | Dynein, axonemal, heavy polypeptide 7 isoform 1 | 232 | 5.5 | 61446 | 5 | 11 |
| HP | gi|149699777 | Haptoglobin precursor | 112 | 5.59 | 38441 | 3 | 9 |
| HPX | gi|149719403 | Hemopexin | 122 | 7.58 | 51324 | 3 | 6 |
| HRG | gi|194222677 | Histidine-rich glycoprotein | 245 | 7.64 | 42955 | 5 | 16 |
| IGHC1 | gi|15020816 | Immunogobulin gamma 1 heavy chain constant region | 31 | 7.68 | 37415 | 1 | 2 |
1 Accession N° is an accession number from the NCBInr databases. 2 Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. 3 PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number. 4 Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.