Table 3.
Identified proteins of mare follicular fluid after enrichment by hexapeptide ligand library and separation by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)
Gene | Accession N° 1 |
Protein name | Score2 | Theoretical | Peptide N°3 | Sequence coverage (%) 4 | |
---|---|---|---|---|---|---|---|
pI 3 | Mr (Da)3 | ||||||
ACT | gi|6626 | Actin | 55 | 5.29 | 41779 | 1 | 2 |
ALB | gi|126723507 | Preproalbumin | 188 | 5.95 | 68554 | 4 | 7 |
ALB | gi|2492797 | Albumin precursor | 28 | 5.85 | 67837 | 1 | 1 |
AMBP | gi|149738520 | AMBP protein precursor | 101 | 6.82 | 38985 | 2 | 5 |
AMY2A | gi|178585 | Alpha-amylase | 78 | 6.32 | 57714 | 1 | 3 |
AMY2A | gi|178585 | Alpha-amylase | 37 | 6.32 | 57714 | 1 | 3 |
AMY2A | gi|178585 | Alpha-amylase | 106 | 6.32 | 57714 | 1 | 3 |
APOA1 | gi|149716548 | Apolipoprotein A-I precursor | 883 | 5.66 | 30312 | 13 | 50 |
APOA1 | gi|149716548 | Apolipoprotein A-I precursor | 675 | 5.66 | 30312 | 13 | 44 |
APOA1 | gi|149716548 | Apolipoprotein A-I precursor | 900 | 5.66 | 30312 | 17 | 62 |
APOA1 | gi|149716548 | Apolipoprotein A-I precursor | 281 | 5.66 | 30312 | 6 | 25 |
C3 | gi|73987236 | Complement C3 precursor | 85 | 6.95 | 173988 | 1 | 1 |
C3 | gi|116597 | Complement C3 precursor | 53 | 6.12 | 186342 | 1 | 0 |
C3 | gi|554423 | Complement component C3 | 179 | 5.73 | 31844 | 3 | 17 |
C3 | gi|554423 | Complement component C3 | 86 | 5.73 | 31844 | 2 | 8 |
CLU | gi|126352584 | Clusterin | 43 | 5.77 | 52121 | 1 | 2 |
CLU | gi|126352584 | Clusterin | 248 | 5.77 | 52121 | 3 | 12 |
CLU | gi|126352584 | Clusterin | 240 | 5.77 | 52121 | 4 | 12 |
FCN1 | gi|194226003 | Ficolin 1 | 156 | 5.37 | 34486 | 4 | 14 |
FGA | gi|3789960 | Fibrinogen A-alpha chain | 65 | 6.45 | 49477 | 1 | 2 |
FGA | gi|3789960 | Fibrinogen A-alpha chain | 65 | 6.45 | 49477 | 2 | 4 |
FGA | gi|3789960 | Fibrinogen A-alpha chain | 695 | 6.45 | 49477 | 10 | 37 |
FGB | gi|149698358 | Fibrinogen beta chain precursor | 502 | 8.53 | 55398 | 6 | 26 |
FGB | gi|149698358 | Fibrinogen beta chain precursor | 409 | 8.53 | 55398 | 8 | 28 |
FGB | gi|149698358 | Fibrinogen beta chain precursor | 137 | 8.53 | 55398 | 2 | 6 |
FGB | gi|149698358 | Fibrinogen beta chain precursor | 125 | 8.53 | 55398 | 2 | 6 |
FGG | gi|1916266 | Fibrinogen-gamma | 69 | 4.78 | 8740 | 2 | 62 |
FH | gi|194227377 | Complement regulator factor H | 95 | 7.52 | 140649 | 2 | 1 |
GAPDH | gi|31645 | Glyceraldehyde-3-phosphate dehydrogenase | 38 | 8.26 | 36031 | 1 | 4 |
GPX | gi|7262515 | glutathione peroxidase | 144 | 5.78 | 8893 | 2 | 37 |
HRG | gi|194222677 | Histidine-rich glycoprotein | 227 | 7.64 | 43003 | 4 | 12 |
PSP | gi|126352355 | Parotid secretory protein | 79 | 4.75 | 26882 | 2 | 9 |
SNX1 | gi|149711176 | Nexin-1 isoform 1 | 75 | 9.53 | 44065 | 1 | 3 |
UN | gi|28317 | Unnamed protein product | 84 | 5.17 | 59492 | 1 | 2 |
1 Accession N° is an accession number from the NCBInr databases.2 Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. 3 PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number4 Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.