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. 2011 Sep 17;9:54. doi: 10.1186/1477-5956-9-54

Table 3.

Identified proteins of mare follicular fluid after enrichment by hexapeptide ligand library and separation by 2D-PAGE (pI: 3-10; MW: 10-200; silver staining)

Gene Accession
1
Protein name Score2 Theoretical Peptide N°3 Sequence coverage (%) 4

pI 3 Mr (Da)3
ACT gi|6626 Actin 55 5.29 41779 1 2
ALB gi|126723507 Preproalbumin 188 5.95 68554 4 7
ALB gi|2492797 Albumin precursor 28 5.85 67837 1 1
AMBP gi|149738520 AMBP protein precursor 101 6.82 38985 2 5
AMY2A gi|178585 Alpha-amylase 78 6.32 57714 1 3
AMY2A gi|178585 Alpha-amylase 37 6.32 57714 1 3
AMY2A gi|178585 Alpha-amylase 106 6.32 57714 1 3
APOA1 gi|149716548 Apolipoprotein A-I precursor 883 5.66 30312 13 50
APOA1 gi|149716548 Apolipoprotein A-I precursor 675 5.66 30312 13 44
APOA1 gi|149716548 Apolipoprotein A-I precursor 900 5.66 30312 17 62
APOA1 gi|149716548 Apolipoprotein A-I precursor 281 5.66 30312 6 25
C3 gi|73987236 Complement C3 precursor 85 6.95 173988 1 1
C3 gi|116597 Complement C3 precursor 53 6.12 186342 1 0
C3 gi|554423 Complement component C3 179 5.73 31844 3 17
C3 gi|554423 Complement component C3 86 5.73 31844 2 8
CLU gi|126352584 Clusterin 43 5.77 52121 1 2
CLU gi|126352584 Clusterin 248 5.77 52121 3 12
CLU gi|126352584 Clusterin 240 5.77 52121 4 12
FCN1 gi|194226003 Ficolin 1 156 5.37 34486 4 14
FGA gi|3789960 Fibrinogen A-alpha chain 65 6.45 49477 1 2
FGA gi|3789960 Fibrinogen A-alpha chain 65 6.45 49477 2 4
FGA gi|3789960 Fibrinogen A-alpha chain 695 6.45 49477 10 37
FGB gi|149698358 Fibrinogen beta chain precursor 502 8.53 55398 6 26
FGB gi|149698358 Fibrinogen beta chain precursor 409 8.53 55398 8 28
FGB gi|149698358 Fibrinogen beta chain precursor 137 8.53 55398 2 6
FGB gi|149698358 Fibrinogen beta chain precursor 125 8.53 55398 2 6
FGG gi|1916266 Fibrinogen-gamma 69 4.78 8740 2 62
FH gi|194227377 Complement regulator factor H 95 7.52 140649 2 1
GAPDH gi|31645 Glyceraldehyde-3-phosphate dehydrogenase 38 8.26 36031 1 4
GPX gi|7262515 glutathione peroxidase 144 5.78 8893 2 37
HRG gi|194222677 Histidine-rich glycoprotein 227 7.64 43003 4 12
PSP gi|126352355 Parotid secretory protein 79 4.75 26882 2 9
SNX1 gi|149711176 Nexin-1 isoform 1 75 9.53 44065 1 3
UN gi|28317 Unnamed protein product 84 5.17 59492 1 2

1 Accession N° is an accession number from the NCBInr databases.2 Score is -10*Log (P), where P is the probability that the observed match is a random event, it is based on NCBInr database using the MASCOT searching program. 3 PI: isoelectric point; Mr: Molecular weight; Peptid N°: Peptid number4 Sequence coverage (%) is based on number of peptide masses matched in NCBInr databases.