Figure 3.

Quantitative PCR results of HES and HEY genes in HemSCs and HemECs. Quantitative PCR results showed that HES1, HEY1, and HEYL had higher transcript levels in HemSCs when compared to HemECs (*p<0.05, #p<0.01.) Gene transcript levels were expressed as relative to beta-actin levels. Results were done in triplicates at least in 2 separate experiments and representative of 2 different cell lines. Statistical analysis showed that the difference in transcript levels for HEY1 approached, but did not achieve, statistical significance (p = 0.08). However, the transcript levels were statistically different between HemSCs and HemECs: Hey2 (p = 0.02), HeyL (p = 0.002), and Hes1 (p = 0.008).