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Effects of temperature and duration of HS on diploid gynogen yield. Eggs activated with UV irradiated sperm were heat shocked starting at 5 mpf at 30°C and 34°C for 2, 4, 6, and 8 minutes. Control 1 and Control 2 served as HS controls activated with UV irradiated sperms 5 mpf and after all the UV treatments, respectively, but not heat shocked. Here, results were presented as means and standard error of the raw data from three independent trials.
