Table 2. T. tenax candidate genes coding for the key enzymes involved in the dicarboxylate/4-hydroxbutyrate cycle.

Enzyme	T. tenax ORF ID	Gene ID best hit aa identity and e-value
PEP-carboxylase (pepC)	TTX_1107	Tneu_0418 (100%, 0.0)
		Igni_0341 (91%, 6e-60)
Succinyl-CoA reductase	TTX_1101	Tneu_0421 (99%, 0.0)
Succinic semialdehyde reductase	TTX_1106	Tneu_0419 (100%, 5e-138)
4-Hydroxybutyrate-CoA ligase (AMP-forming)	TTX_1100	Tneu_0420 (82%, 0.0)
4-Hydroxybutyryl-CoA dehydratase	TTX_1102	Tneu_0422 (100%, 0.0)
Crotonyl-CoA hydratase/(S)-3-Hydroxybutyryl-CoA DH	TTX_1028	Tneu_0541 (99%, 0.0)
		Igni_1058 (99%, 1e-160)
Acetoacetyl-CoA β-ketothiolase	TTX_0886	Tneu_0249 (99%, 1e-166)
		Igni_1401 (99%, 2e-104)

The corresponding e-values derived from blastp analyses of the T. neutrophilus (Tneu_) and I. hospitalis (Igni_) candidates involved in the cycle in these organisms [10], [34], [35] are given.