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. 2011 Oct 7;6(10):e24586. doi: 10.1371/journal.pone.0024586

Table 2. CSP peptides used in ELISpot and ICS assays.

Pool CSP aa # Pep* Class HLA restriction Residues Sequence
1 + 1–39 7 I A2.1 supertype 1–10 MMRKLAILSV
I A2 supertype 7–16 ILSVSSFLFV
II DR (A2.1 and A2 supertype) 1–20 MMRKLAILSVSSFLFVEALF
2 + 29–71 8
3 61–107 9 I B8 81–89 KLRKPKHKK
4 97–283 12 II DR 105–116 NANPNVDPNANP
5 273–319 9 II DR (B7) 281–300 QGHNMPNDPNRNVDENANAN
I B7 86–94 MPNDPNRNV
6 + 309–331 3 I A1 310–319 EPSDKHIKEY
I A2.1 319–327 YLNKIQNSL
II DR (A2.1) 316–335 IKEYLNKIQNSLSTEWSPCS
7 321–335 6 II Th2r 326–343 SLSTEWSPCSVTCGNGIQ
8 345–367 3 II B35-Th3r 346–365 IKPGSANKPKDELDYANDIE
9 + 357–397 8 II DR 363–383 DIEKKICKMEKCSSVFNVVNS
II DR (A2 supertype) 375–397 SSVFNVVNSSIGLIMVLSFLFLN
I A2 supertype 386–394 GLIMVLSFL

PfCSP peptide sequences and residue numbers were based on those of the P. falciparum clone 3D7 (GenBank no. X15363). Previously identified Class I and II CSP epitopes were distributed among peptide pools, except pool 2.

*Number of 15mer peptides in each pool.

+

Peptide pools used in ICS assays.