Table 2. CSP peptides used in ELISpot and ICS assays.
Pool | CSP aa | # Pep* | Class | HLA restriction | Residues | Sequence |
1 + | 1–39 | 7 | I | A2.1 supertype | 1–10 | MMRKLAILSV |
I | A2 supertype | 7–16 | ILSVSSFLFV | |||
II | DR (A2.1 and A2 supertype) | 1–20 | MMRKLAILSVSSFLFVEALF | |||
2 + | 29–71 | 8 | ||||
3 | 61–107 | 9 | I | B8 | 81–89 | KLRKPKHKK |
4 | 97–283 | 12 | II | DR | 105–116 | NANPNVDPNANP |
5 | 273–319 | 9 | II | DR (B7) | 281–300 | QGHNMPNDPNRNVDENANAN |
I | B7 | 86–94 | MPNDPNRNV | |||
6 + | 309–331 | 3 | I | A1 | 310–319 | EPSDKHIKEY |
I | A2.1 | 319–327 | YLNKIQNSL | |||
II | DR (A2.1) | 316–335 | IKEYLNKIQNSLSTEWSPCS | |||
7 | 321–335 | 6 | II | Th2r | 326–343 | SLSTEWSPCSVTCGNGIQ |
8 | 345–367 | 3 | II | B35-Th3r | 346–365 | IKPGSANKPKDELDYANDIE |
9 + | 357–397 | 8 | II | DR | 363–383 | DIEKKICKMEKCSSVFNVVNS |
II | DR (A2 supertype) | 375–397 | SSVFNVVNSSIGLIMVLSFLFLN | |||
I | A2 supertype | 386–394 | GLIMVLSFL |
PfCSP peptide sequences and residue numbers were based on those of the P. falciparum clone 3D7 (GenBank no. X15363). Previously identified Class I and II CSP epitopes were distributed among peptide pools, except pool 2.
*Number of 15mer peptides in each pool.
Peptide pools used in ICS assays.