. 2011 Oct 7;6(10):e24586. doi: 10.1371/journal.pone.0024586

Table 3. AMA1 peptides used in ELISpot and ICS assays.

Pool	AMA1 aa	# Pep^*	Class	T epitope^%	Residues	Sequence
1 ⁺	1–63	13	II	PL186	14–35	EFTYMIFNRGQNYWEHPYQKS
				PL187	41–51	INEHRPKEY
2 ⁺	53–115	13		PL188	92–103	NLFSSIEIVERS
3 ⁺	105–167	13
4 ⁺	157–219	13		PL189	188–204	PLMSPMTLDEMRHFYKD
5	209–271	13		PL190	218–229	SRHAGNMIPDND
				PL191	259–271	NGPRYCNKDE
6	261–323	13		PL192	279–288	AKDISFQNYT
7 ⁺	313–375	13		PL171	348–366	DQPKQYEQHLTDYEKIKEG
8 ⁺	365–427	13		PL193	390–402	YKSHGKGYNWGNY
9	417–479	13		PL172	444–461	SLYKNEIMKEIERESKRI
10 ⁺	469–531	13
11 ⁺	521–583	13		PL194	527–538	EYKDEYADIPEH
				PL173	571–588	GNAEKYDKMDEPQHYGKS ^@
12	573–622	10		PL173	571–588	GNAEKYDKMDEPQHYGKS ^@

AMA1 peptide sequences and residue numbers were based on those of the P. falciparum clone FC27. Gene Bank ID 810891. Previously identified Class II AMA1 epitopes were distributed among peptide pools, except pools 3 and 10.

*Number of 15mer peptides in each pool.

As described in reference 46.

⁺

Peptide pools used in ICS assays.

Epitope PL173 overlaps AMA1 peptide pools 11 and 12.