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. 1984 Jul 11;12(13):5277–5285. doi: 10.1093/nar/12.13.5277

A cytological map of the human X chromosome--evidence for non-random recombination.

D A Hartley, K E Davies, D Drayna, R L White, R Williamson
PMCID: PMC318919  PMID: 6547776

Abstract

The cytological location of six cloned DNA sequences on the human X chromosome has been determined to a high resolution by direct hybridisation 'in situ' to metaphase chromosomes. Each locus has been identified using clones which also detect restriction fragment length polymorphisms by Southern hybridisation. The six loci identified are spaced along the chromosome from Xp22 to Xq28. By combining data obtained using this powerful sequence localisation technique with that from hybrid cell panels and from family studies, it is possible to compare physical and genetic distances, and to demonstrate that the frequency of reciprocal genetic exchange is not uniform along the chromosome length.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Botstein D., White R. L., Skolnick M., Davis R. W. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet. 1980 May;32(3):314–331. [PMC free article] [PubMed] [Google Scholar]
  2. Callan H. G., Perry P. E. Recombination in male and female meiocytes contrasted. Philos Trans R Soc Lond B Biol Sci. 1977 Mar 21;277(955):227–233. doi: 10.1098/rstb.1977.0013. [DOI] [PubMed] [Google Scholar]
  3. Davies K. E., Pearson P. L., Harper P. S., Murray J. M., O'Brien T., Sarfarazi M., Williamson R. Linkage analysis of two cloned DNA sequences flanking the Duchenne muscular dystrophy locus on the short arm of the human X chromosome. Nucleic Acids Res. 1983 Apr 25;11(8):2303–2312. doi: 10.1093/nar/11.8.2303. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davies K. E. The application of DNA recombinant technology to the analysis of the human genome and genetic disease. Hum Genet. 1981;58(4):351–357. doi: 10.1007/BF00282814. [DOI] [PubMed] [Google Scholar]
  5. Davies K. E., Young B. D., Elles R. G., Hill M. E., Williamson R. Cloning of a representative genomic library of the human X chromosome after sorting by flow cytometry. Nature. 1981 Oct 1;293(5831):374–376. doi: 10.1038/293374a0. [DOI] [PubMed] [Google Scholar]
  6. Giraud F., Ayme S., Mattei J. F., Mattei M. G. Constitutional chromosomal breakage. Hum Genet. 1976 Oct 28;34(2):125–136. doi: 10.1007/BF00278880. [DOI] [PubMed] [Google Scholar]
  7. Harper M. E., Saunders G. F. Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization. Chromosoma. 1981;83(3):431–439. doi: 10.1007/BF00327364. [DOI] [PubMed] [Google Scholar]
  8. Hultén M. Chiasma distribution at diakinesis in the normal human male. Hereditas. 1974;76(1):55–78. doi: 10.1111/j.1601-5223.1974.tb01177.x. [DOI] [PubMed] [Google Scholar]
  9. Kirsch I. R., Morton C. C., Nakahara K., Leder P. Human immunoglobulin heavy chain genes map to a region of translocations in malignant B lymphocytes. Science. 1982 Apr 16;216(4543):301–303. doi: 10.1126/science.6801764. [DOI] [PubMed] [Google Scholar]
  10. Laurie D. A., Palmer R. W., Hultén M. A. Chiasma derived genetic lengths and recombination fractions: chromosomes 2 and 9. Ann Hum Genet. 1982 Jul;46(Pt 3):233–244. doi: 10.1111/j.1469-1809.1982.tb00715.x. [DOI] [PubMed] [Google Scholar]
  11. Lebo R. V., Chakravarti A., Buetow K. H., Cheung M. C., Cann H., Cordell B., Goodman H. Recombination within and between the human insulin and beta-globin gene loci. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4808–4812. doi: 10.1073/pnas.80.15.4808. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lyon M. F. Distribution of crossing-over in mouse chromosomes. Genet Res. 1976 Dec;28(3):291–299. doi: 10.1017/s0016672300016980. [DOI] [PubMed] [Google Scholar]
  13. MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
  14. Malcolm S., Barton P., Murphy C., Ferguson-Smith M. A., Bentley D. L., Rabbitts T. H. Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization. Proc Natl Acad Sci U S A. 1982 Aug;79(16):4957–4961. doi: 10.1073/pnas.79.16.4957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mendelsohn M. L., Mayall B. H., Bogart E., Moore D. H., 2nd, Perry B. H. DNA content and DNA-based centromeric index of the 24 human chromosomes. Science. 1973 Mar 16;179(4078):1126–1129. doi: 10.1126/science.179.4078.1126. [DOI] [PubMed] [Google Scholar]
  16. Murray J. M., Davies K. E., Harper P. S., Meredith L., Mueller C. R., Williamson R. Linkage relationship of a cloned DNA sequence on the short arm of the X chromosome to Duchenne muscular dystrophy. Nature. 1982 Nov 4;300(5887):69–71. doi: 10.1038/300069a0. [DOI] [PubMed] [Google Scholar]
  17. Page D., de Martinville B., Barker D., Wyman A., White R., Francke U., Botstein D. Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes. Proc Natl Acad Sci U S A. 1982 Sep;79(17):5352–5356. doi: 10.1073/pnas.79.17.5352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  19. Solomon E., Bodmer W. F. Evolution of sickle variant gene. Lancet. 1979 Apr 28;1(8122):923–923. doi: 10.1016/s0140-6736(79)91398-9. [DOI] [PubMed] [Google Scholar]
  20. Spierer P., Spierer A., Bender W., Hogness D. S. Molecular mapping of genetic and chromomeric units in Drosophila melanogaster. J Mol Biol. 1983 Jul 25;168(1):35–50. doi: 10.1016/s0022-2836(83)80321-0. [DOI] [PubMed] [Google Scholar]
  21. Stahl F. W. Special sites in generalized recombination. Annu Rev Genet. 1979;13:7–24. doi: 10.1146/annurev.ge.13.120179.000255. [DOI] [PubMed] [Google Scholar]
  22. Steinmetz M., Minard K., Horvath S., McNicholas J., Srelinger J., Wake C., Long E., Mach B., Hood L. A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature. 1982 Nov 4;300(5887):35–42. doi: 10.1038/300035a0. [DOI] [PubMed] [Google Scholar]

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