Table 3. Effect of soaking and germination of whole grains on acid and alkaline phytase activity and specific activity*.
Treatments | Acid Phytase activity(unite/g dwt) | Alkaline Phytase activity(unite/g dwt) | L.S.D | Acid Phytase specific activity unit/mg protein | Alkaline Phytase specific activity unit/mg protein | L.S.D |
Raw | ||||||
Dorado | 1.005±0.045a | 0.777±0.071b | 0.1353 | 0.141±0.006a | 0.110±0.01b | 0.01914 |
Shandawee1-6 | 1.016±0.005a | 0.781±0.006b | 0.0530 | 0.116±0.001a | 0.090±0.001b | 0.00183 |
Giza-15 | 1.011±0.011a | 0.797±0.005b | 0.0733 | 0.125±0.001a | 0.10±0.001b | 0.0028 |
Soaking | ||||||
Dorado | 1.020±0.03a | 0.80±0.019b | 0.0120 | 0.124±0.002a | 0.096±0.002b | 0.0044 |
Shandaweel-6 | 1.023±0.007a | 0.788±0.071b | 0.1136 | 0.113±0.001a | 0.088±0.007b | 0.01097 |
Giza-15 | 1.021±0.033a | 0.798±0.003b | 0.0141 | 0.126±0.004a | 0.098±0.004b | 0.0088 |
Germination | ||||||
Dorado | 1.040±0.05a | 0.825±0.005b | 0.0187 | 0.080±0.003a | 0.063±0.001b | 0.0054 |
Shandaweel-6 | 1.020±0.006a | 0.784±0.007b | 0.0526 | 0.061±0.001a | 0.050±0.001b | 0.0024 |
Giza-15 | 1.030±0.040a | 0.798±0.006b | 0.0646 | 0.072±0.001a | 0.055±0.001b | 0.0022 |
*Values are mean of three replicates ±SD, number in the same column or raw followed by the same letter are not significantly different at P<0.05.