Table 2. Strains and plasmids used in this study.
Strains | Description | Source |
S. Typhimurium SL1344 | ||
MB282 | Wild-type, strepR | Gift from S. Falkow |
MB285 | GFP-labeled MB282 via pGT-KAN; strepR gentR | This study |
MB430 | MB282-derivative; Δ agfBA::KanR cassette; kanR | This study |
MB463 | MB282-derivative; Δ agfD::KanR cassette; kanR | This study |
MB664 | MB282-derivative; bcsB-bcsA-yhjQ replaced with a kanR cassette; strepR kanR | This study |
MB683 | MB664-derivative containing pMB682; strepR kanR, gentR | This study |
ℵ8702 | Derivative of SL1344; mlrA::tetAR | [15] |
SL1344K | Derivative of SL1344; rpoS::kan | [16] |
S. Typhimurium ATCC14028 | Wild-type, smooth | Gift from E. Trees |
S. Enteritidis LJH608 | Isolated from outbreak-associated almonds | [11] |
S. Newport 96E01153-C-TX | Isolated from outbreak-associated alfalfa seeds | [51] |
S. Thompson 99A2345 | Clinical isolate from outbreak linked to cilantro in California | [52] |
E. coli K-12 ATCC29425 | Wild-type K-12 | ATCC |
MB684 | Wild-type K-12 ATCC29425 transformed with pMB682; gentR | This study |
E. coli O157:H7 EDL933 | Isolated from outbreak-associated hamburger meat | ATCC |
Pantoea agglomerans | ||
MB42 | Isolated from leaves of cilantro plants in California | [53] |
Pseudomonas chlororaphis | ||
MB10 | Isolated from leaves of cilantro plants in California | [53] |
Aspergillus niger MB7 | Isolated from almond kernels in California | Gift from S. Hua |
Plasmids | ||
pGT-KAN | Broad-range vector with constitutively expressed gfp; gentR | [54] |
pBBR1MCS-5 | Broad-range cloning vector; gentR | [45] |
pMB682 | pBBR1MCS-5 carrying the cellulose operon of SL1344l; gentR | This study |