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. 2011 Oct 7;6(10):e25841. doi: 10.1371/journal.pone.0025841

Figure 2. Ntn1a is necessary to prevent a second CaP/VaP axon from extending beyond the muscle pioneers.

Figure 2

Projected confocal stacks of living dye-labeled motoneurons in one spinal hemisegment. A. VaP axon stalled at muscle pioneers (dashed line) at 36 hpf in standard control-MO injected embryo. B. nt1a SBMO injected embryos had two CaP/VaP axons that extended beyond the muscle pioneers. One CaP/VaP cell also extended an ectopic branch (arrow) along the horizontal myoseptum. B'-B”. The same cells as shown in B. At 25 hpf (B') the red cell was a CaP, but by 31 hpf (B”) the red cell had retracted its axon to the muscle pioneers intermediate target and the green cell had become CaP. Scale bars  = 9 µm in A, B and 20 µm in B', B”.