Skip to main content
. 2011 Oct 7;6(10):e25841. doi: 10.1371/journal.pone.0025841

Table 1. MO sequences used to knock down Netrins, Dcc and Dscam and RT primer sequences used to confirm MO efficacy.

MO MO Sequence (5′-3′) RT-PCR primers (5′-3′) Reference
ntn1a SBMO ATGATGGACTTACCGACACATTCGT ntn1a pre-mRNA (F1 X R1)ntn1a mRNA (F1 X R2)F1: CTTTCGGAGACGAAAACGAGR1: GTAGGCGCTTTCCAGAGATGR2: CTTTGCAGTAGTGGCAGTGG Suli et al. 2007 [17]; Suli 2006 [26]
ntn1a TBMO CGCCTTCCTCAGCCTCTCCTGTGCT n/a n/a
ntn1a 5-mispair MO ATcATGcACTTAgCGACAgATTgGT (lower case letters are mismatches) n/a n/a
ntn1b SBMO TAGTTTAGAAATGACTCACCGACAC ntn1b pre-mRNA (F1 X R1)ntn1b mRNA (F1 X R2)F1: CCGACATCAAAGTGACCTTCR1: GAGCCATCCACACTTGTTGAR2: TGCACGTCGGTGTGATATAG complementary to ntn1b exon 1/ intron 1–2 boundary
ntn2 SBMO TTTCGTGACTTACGTAAGCACTCGT net2E1F: TCCGGAGTGTGATCGATGTAnet2E3R: CCTTTAGCACAGCGGTTACA netrin2 SBMO3, Suli et al., 2007 [17]
dcc TBMO GAATATCTCCAGTGACGCAGCCCAT n/a Suli et al., 2006 [26]
dscam SBMO AAAGATCCTGAAATGCTCACCGGCC dscam pre-mRNA (e2F X i2R)dscam mRNA (e2F X e3R)e2F: TTCTCAGTGAAGACCTACATTCCi2R: ATTCTGGGTAAGAGCTGTGAe3R: AATGCACTTGAAGACCGCTA complementary to dscam exon2/intron 2–3 boundary
dscam TBMO CGCTCCTTTCAATCTCCAAACTAAG n/a DS2M, Yimlamai et al., 2005 [25]
standard control TBMO CCTCTTACCTCAGTTACAATTTATA n/a Gene Toolshttps://store2.gene-tools.com/node/7