Skip to main content
. 2011 Oct 7;6(10):e25875. doi: 10.1371/journal.pone.0025875

Figure 4. JDP2:DBD binding-induced conformational changes facilitate interactions of AF1 with specific coregulatory proteins in cell as assessed by FRET analyses.

Figure 4

A) Representative same-cell images in the donor (CFP-GR500) and YFP-TBP, YFP-CBP, or YFP-SRC-1 channel before and after PB. The areas within the white boxes were photo bleached. 1 and 4 =  CFP- Pre PB; 2 and 5 =  CFP- Post PB; 3 and 6 =  YFP- Post PB. Upper panel, CFP-GR500 and YFP-TBP (left hand); and CFP-GR500 and YFP-TBP in the presence of JDP2 (right hand). Middle panel, CFP-GR500 and YFP-CBP (left hand); and CFP-GR500 and YFP-CBP in the presence of JDP2 (right hand). Lower panel, CFP-GR500 and YFP-SRC-1 (left hand); and CFP-GR500 and YFP-SRC-1 in the presence of JDP2 (right hand). Cells were also cotransfected with a promoter-reporter construct, GRE-SEAP (as described in Materials and Methods). B) Panel displays calculated average FRET efficiencies for each condition. Experiments were carried out three independent times and were analyzed and calculated average FRET efficiencies ± SD of 15 cells were graphed for each of the conditions.