Figure 5. JDP2:DBD interaction-dependent cofactor-binding increases AF1-mediated transcriptional activity of a promoter containing 3xGRE as assessed by SEAP-based promoter:reporter assay.

CV-1 cells cotransfected with vectors containing genes for GR500 with or without other cofactors (as indicated). Results are expressed as means ±SE. Experiments were repeated at least three times. Levels of significance were evaluated by a two-tailed paired Student's t test and P<0.05 was considered significant. Graphs were normalized to transfection efficiency of each construct assayed by immunoblot with specific antibody to GR (lower panel).