Figure 1. IRF4 drives the differentiation of myeloid progenitors towards macrophages.

(A) Wright-Giemsa stain of Tot2 cells transduced with empty MSCV-puro, MSCV-IRF4FLAG-puro or MSCV-IRF8FLAG-puro (original magnification, x 600). (B) Surface marker analysis. Cells on day 6 were stained with the indicated antibodies or isotype control antibodies and analyzed by flow cytometry. Note that differentiated macrophages have higher autofluorescence than undifferentiated cells. (C) Phagocytic activity. Cells on day 6 were incubated with fluorescein-labeled E. coli bioparticles at 37°C or 4°C for 2 h and analyzed by flow cytometry. (D) Immunoblotting analysis of FLAG-tagged IRFs. β-tubulin expression is shown as a loading control.