Figure 2. IRF4 induces macrophage-related genes and growth arrest during macrophage differentiation.

(A) Induction of macrophage-related genes. Transcript levels in MSCV-transduced cells on day 5 were analyzed by qRT-PCR in triplicate. Data were analyzed using the ΔΔCT method and normalized by the Gapdh levels and shown as values relative to those in empty vector-transduced cells (mean ± standard deviation; representative of three independent experiments with similar results). *P<0.01 (Student's t-test). (B) Total viable cell yields (left panel) and cell cycle profiles (on day 4, right panel) after the transduction of MSCVs. Data are expressed as mean ± standard deviation of three independent experiments. *P<0.01 (Student's t-test).