Figure 4. Specific activities of IRF4 and IRF8 in macrophages.

(A) Genes specifically induced by IRF4 or IRF8. Tot2 cells transduced with empty MSCV-puro, MSCV-IRF4FLAG-puro or MSCV-IRF8FLAG-puro were analyzed by qRT-PCR on day 3 (mean ± standard deviation). Data are representative of two independent experiments with similar results. (B) Distinct patterns of cytokine gene induction in IRF4- and IRF8-transduced macrophages. Cells transduced with MSCVs were stimulated on day 5 with 1 µg/ml LPS or 1 µg/ml CpG-B for 5 h, and analyzed by qRT-PCR using the ΔΔCT method (mean ± standard deviation). Two repeat experiments gave similar results. *P<0.01 (Student's t-test).