Table 2. Retention time (Rt), wavelengths of maximum absorption in the visible region (λmax), mass spectral data, tentative identification and total anthocyanin content in the three strawberry cultivars in study.
Peak | Rt (min) | λmax (min) | Molecular ion | Tentative identification | Total anthocyanin content (mg/Kg of FW) | |||
[M+] (m/z) | MS2 (m/z) | Sveva | Alba | Adria | ||||
1 | 21.7 | 515 | 705 | 543,407, | (Epi)afzelechin-(4→8)Pg 3- | nd | nd | 2.73±0.1 |
313,271 | glucoside | |||||||
2 | 23.2 | 515 | 449 | 287 | Cy-3-glucoside | 16.35±5.6 | 19.02±3.5 | 28.97±4.6 |
3 | 24.1 | 500 | 595 | 433, 271 | Pg 3,5-diglucoside | nd | nd | 0.438±0.01 |
4 | 27.3 | 502 | 433 | 271 | Pg 3-glucoside | 611.18±25.5 | 507.51±25.4 | 1054.3±67.4 |
5 | 29.3 | 503 | 579 | 433, 271 | Pg 3-rutinoside | nd | 20.89±4.1 | 101.03±21.5 |
6 | 33.7 | n.a | 535 | 287 | Cy 3-malonylglucoside | nd | 1.17±0.01 | nd |
7 | 37.7 | 504 | 519 | 271 | Pg 3-malonylglucoside | 2.85±0.4 | 65.48±5.6 | 2.350±0.2 |
8 | 40 | 504 | 475 | 271 | Pg 3-acetylglucoside | 7.250±0.2 | 1.13±0.01 | nd |
9 | 41.8 | 508 | 533 | 271 | Pg 3-succinylglucoside? or | nd | nd | 3.83±0.03 |
Pg 3-methylmalonylglucoside? | ||||||||
Total anthocyanin content | 637.63±65.8 | 615.2±53.8 | 1193.64±101.4 |
Values expressed as means (mg/kg of FW) ± SD. nd, not detected.