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. 2011 Oct 7;6(10):e25958. doi: 10.1371/journal.pone.0025958

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Zhang et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Panels A-C are representative GelStar-stained agarose gels of cDNA products resulting from competitive RT-PCR analysis of the SMMHC pre-mRNA of the 5’ region containing the SM-A/SM-B alternative splice site, the 17 kDa essential MLC pre-mRNA of the 3’ region containing the MLC_17a/MLC_17b alternative splice site and the SMMHC pre-mRNA 3’ region containing the SM1/SM2 alternative splice site from sham and PBOO rats, respectively. Panel D shows averaged quantitative determination of SMM pre-mRNA mean isoform percentages in detrusor determined by using the information gathered from gels as in panels A–C. Values are expressed as mean ± SEM. * = p<0.05 vs PBOO; ** = p<0.01 vs PBOO. (n = 5 different animals for each group).