Figure 6. Mesenchymal stem cell conditioned medium or FXN-transfection increases resistance to hydrogen peroxide mediated toxicity in fibroblasts derived from FRDA patients.

The effect of hydrogen peroxide (600 µM) on FRDA and control fibroblast cell survival in vitro post exposure to minimal medium (MIN) or MSC-conditioned medium (MSC CM) for 24hours, with/without the addition of the catalase inhibitor 3-AminoTriazole (CATIN), catalase (CAT) and glutathione peroxidase (GPX1). FXN indicates fibroblasts have been transfected with the GFP-tagged FXN gene. Cell survival was assesed using the MTT cell viability assay (MTT). Cell survival is expressed as a percentage of cell survival compared to cells grown in minimal medium alone. Results are expressed as the mean +/− (SEM). (*p<0.05, comparing test condition to control; n = 3 independent experiments).