Figure 2. Hif1α increases transcription of the CHD4 and MTA3 genes.

(A) MCF-7 cells stably expressing either a non-specific shRNA (shRNA^Con) or shRNA targeting Hif1α (shRNA^Hif1α) were exposed to DMOG (1 mM) or CoCl₂ (200 µM) for 24 hrs. Cell extracts were prepared and then examined by western blot (WB) analysis to monitor levels of Hif1α. β-actin levels are shown to demonstrate equal loading. (B–E) MCF-7 cells stably expressing either a non-specific shRNA (shRNA^Con) or shRNA targeting Hif1α (shRNA^Hif1α) were exposed to solvent (open bars) or CoCl₂ (200 µM; filled bars) for 8 hr. mRNA levels for the indicated genes were measured by RT-PCR as described in methods. Results ± SE (n = 3). (F) MCF7 cells stably expressing either a non-specific shRNA (shRNA^Con) or shRNA targeting Hif1α (shRNA^Hif1α) were exposed to CoCl₂ (200 µM) for indicated number of hours. Cell extracts were prepared and then examined by western blot (WB) analysis to monitor levels of CHD4, MTA3 and Hif1α. β-actin levels are shown to demonstrate equal loading.