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. 2011 Oct 7;6(10):e25994. doi: 10.1371/journal.pone.0025994

Figure 6. Attenuation of gp120-induced neuronal apoptosis by T140 or 4-AP.

Figure 6

A shows immunocytochemical analysis of apoptosis (TUNEL staining) in cortical neuronal cultures induced by gp120 (200 pM) in the absence and presence of T140 (50 nM) or 4-AP (5 mM). Intact cell nuclei were visualized with DAPI staining (blue) of nucleic acids and apoptotic cells were labeled with TUNEL staining (green) of fragmented DNA (magnification ×40). B is a bar graph illustrating the percentage of TUNEL positive cells in response to gp120, T140 and 4-AP and showing that cultures incubated with gp120 for 24 h exhibited a significant increase of apoptotic neurons and that application of T140 or 4-AP significantly attenuated the gp120-induced increase of neuronal apoptosis. 12 randomly selected visual fields were counted in each group ** p<0.01, *** p<0.01. a; 200 pM; b; 500 pM.