Fig. 7.

Acetylation of Lys145 of cyclophilin D modulates enzyme activity and interaction with ANT1. (A) HeLa cells overexpressing wild-type cyclophilin D, cyclophilin D (K145Q) or cyclophilin D (K145R) were transferred to DMEM medium containing glucose or galactose. Recombinant cyclophilins were isolated using anti-FLAG antibody for immunoprecipitation. The peptidyl-prolyl cis-trans isomerase activity of the cyclophilins was determined. The values are the means from triplicate samples, and the error bars indicate s.d. (B) Mitochondrial extracts were immunoprecipitated with anti-ANT1 or anti-FLAG antibody and immunoblotted with anti-FLAG or anti-acetylated-lysine antibody, respectively.