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. Author manuscript; available in PMC: 2012 Apr 1.
Published in final edited form as: Nat Nanotechnol. 2011 Sep 11;6(10):658–667. doi: 10.1038/nnano.2011.105

Figure 3. Competition and dissociation assays of 3WJ-pRNA.

Figure 3

a, Temperature effects on the stability of 3WJ-pRNA core, denoted [ab*c]3WJ evaluated by 16% native gel. Fixed concentration of Cy3 labeled [ab*c]3WJ was incubated with varying concentration of unlabeled b3WJ at 25°C, 37°C, and 55°C. b, Urea denaturing effects on the stability of [ab*c]3WJ evaluated by 16% native gel. Fixed concentration of labeled [ab*c]3WJ was incubated with unlabelled b3WJ at ratios of 1:1 and 1:5 in the presence of 0–6M urea at 25°C. c, Dissociation assay for the [32P]-3WJ-pRNA complex harboring three monomeric pRNA by 2-fold serial dilution (lanes 1–9). Monomer unit is shown on the left.