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. 2001 Apr 10;98(8):4675–4680. doi: 10.1073/pnas.081075298

Figure 2.

Figure 2

Palmitoylation of LMP1 and LMP2A. (A) 293T or BJAB cells were transiently transfected with pSG5 (Vector), pSG5 Flag-LMP1 (F-LMP1), or pLMP2AHA expression vectors and labeled with [3H]palmitate. The immunoprecipitates with anti-Flag M2 affinity gel or anti-HA antibody were analyzed by fluorography. (B) Wild-type LCLs (LMP1) or Flag-LMP1 expressing LCLs (F-LMP1) were labeled with [3H]palmitate, immunoprecipitated with M2 gel, and analyzed by fluorography (Upper). Ten percent of each immunoprecipitate was probed with LMP1 antibody (Lower).