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. 2001 Apr 10;98(8):4675–4680. doi: 10.1073/pnas.081075298

Figure 6.

Figure 6

Association of LMP1 mutants with lipid rafts and cytoskeleton. (A) Triton X-100 (0.2%) lysates from BJAB cells or cells stably expressing wild-type LMP1, PQAA, or ID mutant were subjected to sucrose gradient centrifugation, and aliquots of the peak lipid raft (r), soluble (s), and pellet (p) fractions were probed with anti-LMP1, TRAF3, TRAF2, or TRAF1 antibodies. (B) Gradient fractions from wild-type, 1–231, or 1–231 PQAA LMP1-expressing cells were immunoprecipitated with M2 gel and probed with Flag antibody. Arrows indicate wild-type or mutant LMP1. Ig heavy chain is indicated by asterisk. (C) Lysates from LMP2A null mutant, D1LMP1-expressing ES5 cells were subjected to sucrose gradient centrifugation, and the fractions were probed with LMP1 antibody.