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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Int J Cancer. 2011 Apr 20;130(3):532–543. doi: 10.1002/ijc.26010

Fig. 6. RCAS1 is localized to the ER and is required for VEGF secretion.

Fig. 6

Fig. 6

(A) Real time PCR analysis of RCAS1 expression in total RNA isolated from ES2 cells transduced with an Akt3 shRNA or scrambled control. Expression is shown relative to S26 as an internal control. (B) Western blot analysis of RCAS1 expression in cells treated either with shAkt3 (top) or with Akt3 RNAi (bottom). Relative ratios are shown. (C) Fluorescent images of ES2 cells stained using antibodies against KDEL (green) and RCAS1 (red) and the resultant merged images. (D) RTPCR of RCAS1 and Akt3 expression in cells transfected with either an empty vector control or two different amounts of an Akt3 expression vector. Relative ratios of both RCAS1 and Akt3 are shown. (D) Western blot analysis of VEGF from conditioned media derived from cells ES2 cells transfected with an RNAi directed against RCAS1 or a scrambled control. p85 is shown as a loading control and relative ratios are shown. (E) Western blot analysis of VEGF in conditioned media from ES2 cells transfected with an Akt3 expression vector with or without co-transfection of an RCAS1 RNAi. p85 is shown as an internal control and relative ratios are shown.