Skip to main content
. Author manuscript; available in PMC: 2012 Oct 1.
Published in final edited form as: Cancer Epidemiol Biomarkers Prev. 2011 Aug 22;20(10):2237–2249. doi: 10.1158/1055-9965.EPI-11-0548

Figure 1. Heterogeneity among H. pylori strains in expression of CagA.

Figure 1

A. H. pylori strains were cultured in broth and lysed as described in the Methods. Cell extracts were standardized by protein concentration and immunoblotted to detect CagA. Blots then were stripped and reprobed with an anti-H. pylori antiserum. B. H. pylori 26695 cagA::catrdx-9 was transformed with 1.2 kb DNA fragments derived from the indicated Colombian strains (500 bp upstream and 700 bp downstream of the cagA transcriptional start site). The resulting transformants were cultured as described in the Methods, and were analyzed for CagA expression as described above. C. Analysis of transformants depicted in panel B. Nucleotide numbers are relative to the cagA transcriptional start site, and designate the chromosomal region in H. pylori 26695 that was replaced with sequences derived from Colombian strains. Vertical lines indicate the cagA transcriptional start site and the ATG translation initiation site. D. This schematic illustrates the predicted cagA transcriptional start site (TS), an AT-rich inverse repeat identified in the cagA promoter region of H. pylori 26695, and the ATG translation initiation site.