Table 1.
Expression of CagA in Colombian H. pylori strains
| strain | CagA expressiona |
Number of AATAAGATA motifsb |
Regionc | MLST typed | Histopathology scoree |
|---|---|---|---|---|---|
| 5001 | 1.0 | 0 | low | hpAfrica1 | 2.3 |
| 5004 | 1.4 ± 0.2 | 0 | low | hpAfrica1 | 2.3 |
| 5005 | 0.4 ± 0.2 | 0 | low | hpAfrica1 | 1.7 |
| 5007 | 1.4 ± 0.3 | 0 | low | hpAfrica1 | 2.0 |
| 5009 | 1.4 ± 0.2 | 1 | low | hpEurope | 1.7 |
| 5010 | 0.5 ± 0.3 | 0 | low | hpAfrica1 | 2.0 |
| 5016 | 1.8 ± 0.2 | 0 | low | hpAfrica1 | 3.5 |
| 5017 | 0.9 ± 0.3 | 0 | low | hpAfrica1 | 2.3 |
| 5018 | 1.6 ± 0.5 | 1 | low | hpAfrica1 | 3.5 |
| 5022 | 0.9 ± 0.3 | 0 | low | European | 4.5 |
| 5024 | 1.9 ± 0.1 | 0 | low | hpAfrica1 | 2.0 |
| 5028 | 1.1 ± 0.4 | 1 | low | hpEurope | 3.8 |
| 5032 | 2.3 ± 0.2 | 1 | low | hpEurope | 3.5 |
| 5038 | 3.2 ± 0.5 | 1 | low | hpEurope | 3.5 |
| 5043 | 3.7 ± 0.7 | 1 | low | hpEurope | 5.3 |
| 5051 | 2.4 ± 0.2 | 1 | low | hpAfrica1 | 2.0 |
| 5054 | 1.6 ± 0.6 | 0 | low | hpAfrica1 | 2.3 |
| 5056 | 4.0 ± 0.8 | 1 | high | hpEurope | 4.5 |
| 5057 | 2.4 ± 0.4 | 1 | high | hpEurope | 3.5 |
| 5068 | 2.4 ± 0.4 | 1 | high | hpEurope | 4.3 |
| 5069 | 3.5 ± 0.6 | 1 | high | hpEurope | 4.3 |
| 5072 | 0.7 ± 0.2 | 1 | high | hpEurope | 3.5 |
| 5074 | 5.1 ± 1.1 | 1 | high | hpEurope | 4.7 |
| 5080 | 0.5 ± 0.3 | 1 | high | hpEurope | 3.5 |
| 5082 | 4.4 ± 1.3 | 1 | high | hpEurope | 4.8 |
| 5086 | 4.8 ± 1.1 | 1 | high | hpEurope | 4.6 |
| 5090 | 1.0 ± 0.2 | 0 | high | hpEurope | 2.0 |
| 5091 | 1.9 ± 0.2 | 1 | high | hpEurope | 4.5 |
| 5093 | 3.5 ± 0.3 | 1 | high | hpEurope | 4.5 |
| 5095 | 2.0 ± 0.2 | 1 | high | hpEurope | 2.0 |
| 5096 | 1.5 ± 0.1 | 0 | high | hpEurope | 4.3 |
| 5097 | 1.0 ± 0.3 | 1 | high | hpEurope | 4.4 |
| 5100 | 2.2 ± 0.1 | 1 | high | hpEurope | 2.3 |
| 5104 | 4.7 ± 0.6 | 1 | high | hpEurope | 3.5 |
| 5112 | 2.2 ± 0.1 | 1 | high | hpEurope | 4.3 |
| 5114 | 0.9 ± 0.3 | 0 | high | hpEurope | 4.4 |
CagA expression was analyzed by immunoblotting, and relative levels of expression were quantified by comparing the CagA signal intensity of each strain with that of a standard (CagA from H. pylori strain 5001). For each strain, the CagA signal was calculated for 3 independent biological samples, and the average of these values is presented. Values represent mean ± SEM.
The number of AATAAGATA motifs upstream of the ATG translation initiation site is shown.
H. pylori strains were isolated from regions of Colombia with either high risk or low risk for the development of gastric cancer.
MLST analysis (31) was previously used to characterize the ancestral origin of the H. pylori strains. hpAfrica1 denotes isolates classified as either West African (hspWAfrica) or South African (hspSAfrica).
Histopathology scores describing the severity of lesions observed in gastric tissue from which the H. pylori strains were isolated.