Table 1.
Identification of C. crescentus proteins with differential cell cycle expression profiles
| ORF | CL | Function |
|---|---|---|
| Amino acid metabolism | ||
| 03246* | 16 | Tetrahydropteroyltriglutamate methyltransferase (Met) |
| 04899* | 3 | Glutamyl-tRNA(Gln)-amidotransferase subunit A (Glu) |
| 05974 | 19 | Indole-3-glycerol-phosphate synthase (Trp) |
| 06643 | 19 | Acetylornithine aminotransferase (Arg) |
| Carbohydrate metabolism | ||
| 03310† | 1 | Acetyl-CoA-acetyltransferase |
| 03861† | 7 | β-d-glucoside glucohydrolase |
| 06912* | 7 | UDP-glucose-4-epimerase |
| Cofactor metabolism | ||
| 03206 | 6 | GTP cyclohydrolase I (tetrahydrofolate synthesis) |
| 04038* | 6 | Riboflavin specific deaminase |
| 04041* | 6 | Riboflavin synthase α-chain |
| 04042 | 6 | GTP cyclohydrolase II (riboflavin synthesis) |
| 04043* | 6 | Riboflavin synthase β-chain |
| Lipid metabolism | ||
| 02302 | 3 | Enoyl-CoA-hydratase |
| 02436† | 7 | Fatty acid oxidation complex α-subunit |
| Energy metabolism | ||
| 01686 | 22 | ATP-synthase-α-subunit |
| 02110 | 22 | Aconitase |
| 06056* | 23 | NADH dehydrogenase I chain G |
| 06255 | 22 | Pyruvate kinase |
| Redox reactions | ||
| 00532 | 21 | Thioredoxin reductase |
| 00952† | 3 | Glutathione-S-transferase |
| 01378* | 1 | NADPH-dependent quinone oxidoreductase |
| 02058* | 11 | Glutathione-S-transferase |
| 02406* | 1 | NAD(P)H Nitroreductase |
| 02506* | 1 | Thioredoxin |
| RNA and protein synthesis | ||
| 02595 | 23 | Ribonuclease PH |
| 03553† | 14 | 50S Ribosomal protein L1 |
| 04420 | 9 | Peptide chain release factor 3 |
| 04755† | 6 | 50S Ribosomal protein L4 |
| 05538* | 23 | 50S Ribosomal protein L9 |
| Protein folding and degradation | ||
| 00474 | 1, 22 | Aminopeptidase |
| 00833* | 21 | Peptidase |
| 01270† | 19 | Peptidase |
| 01800* | 1, 7 | Endopeptidase |
| 01944 | 1 | Zinc-metalloprotease |
| 02143† | 2 | Prolyl-endopeptidase |
| 02145 | 3 | Prolyl-endopeptidase |
| 02174* | 7 | Peptidyl-dipeptidase |
| 03639* | 8 | Chaperone GroEL |
| 03641* | 8 | Chaperone GroES |
| 06085* | 12 | Protease ClpP |
| 06128* | 7 | Dipeptidyl-peptidase |
| 07050* | 23 | Amidohydrolase |
| Cell envelope synthesis and structure | ||
| 01771 | 2 | Penicillin binding protein |
| 05095† | 1 | 2-Dehydro-3-deoxyphosphooctonate aldolase |
| Transport protein | ||
| 01077* | 13 | TonB-dependent iron uptake receptor |
| 01388* | 23 | ABC transporter ATP binding protein |
| 01790 | 7 | TonB-dependent iron uptake receptor |
| 05691† | 2 | TonB-dependent receptor |
| Motility and chemotaxis | ||
| 03149* | 13 | Chemotaxis protein CheYI |
| 03156* | 11 | Chemotaxis protein CheR |
| 03157* | 13 | Chemotaxis protein CheB |
| 03161* | 13 | Chemotaxis protein CheD |
| 05156* | 13 | Flagellin FljL |
| 05158* | 4 | Flagellin FljK |
| Cell division | ||
| 07201* | 20 | Cell division protein FtsZ |
| DNA metabolism | ||
| 02197† | 23 | dUTP nucleotidohydrolase |
| 03051* | 13 | DNA methyltransferase CcrM |
| 05175* | 21 | Single strand binding protein SSB |
| Regulatory protein | ||
| 00839* | 15 | Cell cycle transcriptional regulator CtrA |
| 01137* | 23 | Transcriptional regulator of AsnC/Lrp family |
| 02400† | 22 | Ferric uptake regulation protein Fur |
| 02881* | 1 | Phosphate transport regulator PhoU |
| 05154* | 10 | Regulator of flagellin expression FlbT |
| 05981* | 17 | SOS response repressor LexA |
| 06096* | 5 | Nitrogen regulatory protein PII |
| 07059* | 12 | Response regulator PleD |
| 07061* | 13 | Response regulator DivK |
| Unknown function | ||
| 01570* | 1 | Conserved unknown |
| 02168* | 21 | Unknown |
| 02609* | 21 | Unknown |
| 03189* | 12 | Conserved unknown |
| 03651* | 10 | Unknown |
| 03910† | 22 | Conserved unknown |
| 04449 | 21 | Unknown |
| 05475† | 10 | Unknown |
| 05886* | 12 | Conserved unknown |
| 05929* | 7 | Conserved unknown |
| 06393* | 1 | Conserved unknown |
| 06615* | 2 | Conserved unknown |
| 07094 | 12 | Unknown |
| 07198 | 22 | Conserved unknown |
ORF, Caulobacter genome ORF number (see Materials and Methods); *, similar; †, no or inverse cell cycle oscillations were detected for the corresponding mRNAs in ref. 4; CL, cluster number of coexpression groups (Fig. 2); Function, putative function determined by blast homology searches. Proteins with a half life shorter than one cell cycle are shown in bold.