. Author manuscript; available in PMC: 2011 Oct 9.

Published in final edited form as: Pediatr Nephrol. 2010 Jan 5;25(6):1005–1016. doi: 10.1007/s00467-009-1392-6

Table 2.

Gene expression profiling of murine models of kidney dysgenesis

Mutant strain (gene)	Source/species	Technology^a	Outcome	Reference
Megalin ^−/−	12- to 16-week-old mice kidneys	Affymetrix MullK chip B	Megalin regulates vitamin D homeostasis	[59]
Meprin beta^−/−	Adult male mouse kidneys	Affymetrix MG-U74Av2 oligonucleotide array	Changes in genes associated with immune response and cell cycle, increase in TRAF6, Tlr6	[60]
Aqp1^−/−	10-week-old mouse inner medulla	NIA mouse 15 K clone set GRL array	Downregulation of many medullary genes involved in heatshock/stress, mitochondrial pathways, vasopressin receptors	[61]
Gata3^−/−	E9.5 mouse embryos	NIA mouse 15 K cDNA array	Downregulated Dad2	[21]
Pod1^−/−	E18.5 mouse isolated glomeruli	Affymetrix GeneChip Mouse Expression Array 430 2.0	Almost 4000 differentially expressed genes. Fourfold reduction in alpha 8 integrin	[23]
KininB1 and kininB2^−/−	12- to 16-week-old male mouse kidneys	Agilent oligonucleotide arrays	Large number of downregulated genes that are already implicated in renal physiology or pathology	[62]
Pax 2^lacZ/+ Pax 2^GFP	GFP Pro/mesonephric cells	cDNA microarrays	Gata3 in the nephric duct is regulated by Pax2 and Pax8	[63]
Sprague Dawley Cy^−/−	3-week-old rat total kidney	Agilent 60-mer Rat Array G4130A	Upregulation of extracellular matrix metabolism genes in PKD model	[64]
Hoxd 11^−/− Hoxd 11^−/−	E11.5, E12.5, E13.5 and E16.5 mouse kidneys	Affymetrix GeneChip Mouse Expression Array 430A&B	Upregulation of Irx3, downstream target of Hox; changes in, for example, Hoxa11s, GATA6, Slc40a1, Cxcl12; multiple downstream pathways	[65]
c-maf^−/−	Mouse kidneys	Affymetrix GeneChip U74A array	Down-regulation of GPx3 in knockout mice, possibly regulated by c-maf	[66]
Pygo1^−/−/ Pygo2^−/−	El8.5 mouse kidneys	Illumina Sentrix Mouse-6 expression Beadchip	Expression changes in candidate Wnt signaling targets but Pygol/Pygo2 not essential for canonical Wnt signaling.	[67]
Rarb2Cr^tg/+; Lim1^lz/flox	LCM isolated renal vesicles	Affymetrix GeneChip Mouse Expression Array 430 2.0	Differential expression of Chrdl2, Bmf, myob5, pdgfrl and 9× downregulation of Dkk1	[24]
Aqp 1^−/−	8-week-old mouse renal medulla ± infusion of vasopressin	cDNA microarrays	Increased cyclin Dl and Egr accompanied by increased cell proliferation	[68]
Hnf1 beta^−/−	mIMCD3 cells	CHIP-chip & Affymetrix GeneChip Mouse Expression Array 430A&B	Hnf-1 beta binds to and regulates Socs3 to control tubulogenesis	[22]
Nephrin^−/−	E18.5 glomeruli mouse	Affymetrix GeneChip Mouse Expression Array 430 2.0	Nephrin not essential for cell survival but plays a role in glomerular differentiation and development.	[69]
Glis2^mut	PND25 & PND60 total kidney	Agilent whole-mouse genome microarrays	Upregulation of genes involved in ECM homeostasis and immune/inflammatory response	[70]
Wnt4 n/+	E14.5 mouse kidney	Affymetrix GeneChip MG-U74Av2 arrays	>50% genes expressed in renal vesicle or later stage	[71]
Aqp 11^−/− and Pkd1^−/−	PND1 mouse kidneys	Affymetrix GeneChip Mouse Expression Array 430 2.0	Comparison suggests strong similarities between the two strains (same changes in Myc, Egfr, Egf, Mmp12, Timp1, Tgfb1)	[72]
NPHS2-cre; Dicer^Flx/Flx	3-week-old mouse isolated glomeruli	Exiqon's miRCURY LNA array	mmu-miR-23b, mmu-miR-24, and mmu-miR-26a identified in glomeruli	[27]
NPHS2-cre; Dicer^Flx/Flx	4- to 5-week old mouse glomeruli	Affymetrix GeneChip Mouse Expression Array 430 2.0	Upregulation of podocyte specific members of mir-30 miRNA family	[28]

PND, Post-natal day; GFP, green fluorescent protein; PKD model, polycystic kidney disease model; ECM, extracellular matrix

Exiqon, Vedbaek, Denmark; Agilent Technologies, Santa Clara, CA; Illumina, San Diego, CA