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. Author manuscript; available in PMC: 2012 Sep 28.
Published in final edited form as: J Am Chem Soc. 2011 Sep 6;133(38):15139–15147. doi: 10.1021/ja2055098

Figure 2.

Figure 2

Reversed-phase HPLC Analysis of Transferase Reactions. Top: AaT-mediated transfer of Phe from adenosyl donor 4a to LysAlaAcm reporter peptide. HPLC chromatograms obtained after (A) 0 h or (B) 4 h show conversion of LysAlaAcm (11.8 min retention time) to PheLysAlaAcm (13.0 min retention time). Conversion after one addition of donor 4a is 92 % (B). A second addition of 1 mM 4a drives the reaction to completion with a small amount of double Phe addition (C). Bottom: HPLC analysis of transfer of Nap from adenosyl donor 4d to LysAlaAcm after (D) 4 h shows conversion to NapLysAlaAcm (14.1 min retention time). Product identities confirmed by observation of indicated masses by MALDI MS.