Figure 1.
Interaction of the different hybrid proteins with purified insoluble chitin. Binding assay mixtures contained 10 mg (dry weight) of insoluble chitin and 25 pmol of protein in 500 μL of 50 mM sodium phosphate buffer, 150 mM NaCl (pH 7.4). Assay mixtures were incubated for 2 h at room temperature for binding. After removing the supernatant, the pellet was washed three times and incubated for 2 min with 1 mL of nitrocefin (100 μM). After filtration of the suspension, the absorbance of the filtrate was measured at 482 nm. This experiment has been repeated three times (each in duplicates). The bars represent the averages ± SD of these independent experiments. Error propagation was used to normalize the interaction of the different chimeric proteins with their respective kcat.