Figure 3.

Interaction of BlaPChBDI with fungal chitin cell wall after treatment with zymolyase, PNGase, and Type XIV protease. In this experiment, the washed cells were incubated in the presence of the different hydrolytic enzymes before incubation with the chimeric proteins. After this treatment, cells were successively washed twice with 50 mM sodium phosphate buffer, incubated with the different hybrid proteins, washed again, and incubated with nitrocefin as described above. Finally, absorbance of the filtrate was measured at 482 nm. This experiment has been repeated three times (each in duplicates). The bars represent the averages ± SD of these independent experiments.