Table 2.
Differentially expressed actin cytoskeleton-associated proteins in glomeruli isolated from OLETF and LETO rats, identified by QSTAR Elite LC-MS/MS and IPA.
| Protein | GI number | Mass (Da) | Location | Function | Foldchange | P value | Fold change | P value |
|---|---|---|---|---|---|---|---|---|
| (27 weeks) | (38 weeks) | |||||||
| Stress fiber formation | ||||||||
| Sorbin and SH3 domain containing 2 (SORBS2) | 205831248 | 124108 | C | AD | 1.49 | <0.0001 | 1.97 | <0.0001 |
| Alpha-actinin 1 (ACTN1) | 13123942 | 103058 | C | ST, CL | 1.02 | 0.764 | 1.40 | <0.0001 |
| Alpha-actinin 4 (ACTN4) | 182705246 | 104654 | C | ST, CL | 0.93 | 0.46 | 1.22 | <0.0001 |
| Rho GDP dissociation-inhibitor alpha (ARHGDIA) | 21759130 | 23207 | C | RI | 1.28 | 0.041 | 0.87 | 0.0413 |
| Actin-filament polymerization | ||||||||
| Actin-related protein 2/3 complex subunit 1 beta (ARPC1B) | 12229626 | 40950 | C | AP, EC | 0.65 | 0.0363 | 0.73 | 0.0136 |
| Actin related protein 2/3 complex subunit 5 (ARPC5) | 3121767 | 16320 | C | AP, EC | 0.72 | 0.0014 | 0.74 | 0.0002 |
| Myristoylated alanine-rich protein kinase C substrate (MARCKS) | 266495 | 31555 | UN | AP | 0.60 | 0.0004 | 0.71 | 0.002 |
| Microtubules formation | ||||||||
| Tubulin alpha 1c (TUBA1C) | 55976169 | 49895 | C | MT | 0.74 | 0.012 | 0.90 | 0.17 |
| Intermediate filaments formation | ||||||||
| Lamin A/C (LMNA) | 1346413 | 74139 | N | IF | 0.89 | 0.0076 | 1.29 | <0.0001 |
| Desmin (DES) | 1352241 | 53536 | C | IF | 0.94 | 0.143 | 1.32 | <0.0001 |
| Nestin (NES) | 146345465 | 177439 | C | IF | 0.83 | <0.0001 | 1.03 | 0.32 |
| Plectin 1 (PLEC1) | 1709655 | 531791 | C | IL | 0.82 | <0.0001 | 0.96 | 0.12 |
| GBM (glomerular basement membrane) | ||||||||
| Integrin beta 1 (INTGB1) | 1352494 | 15105 | PM | APG | 0.82 | 0.0015 | 0.92 | 0.0094 |
| Agrin (AGRN) | 399021 | 214846 | PM | LB | 0.62 | 0.042 | 0.90 | 0.490 |
| Others | ||||||||
| Plastin 3 (PLS3) | 226693553 | 70811 | C | CL | 0.82 | 0.02 | 0.82 | 0.0005 |
| Calponin 3 (CNN3) | 584956 | 36414 | C | AB | 0.60 | 0.0013 | 0.78 | 0.088 |
| Tropomyosin 3 (TPM3) | 148840439 | 32819 | C | AS | 0.71 | <0.0001 | 0.80 | <0.0001 |
AB: actin binding; AD: adaptor protein; AP: actin filament polymerization; APG: anchoring podocyte and GBM (glomerular basement membrane); AS: actin filaments stabilization; C: cytoplasm; CL: crosslinking actin filaments into bundles or networks; EC: endocytosis; IF: intermediate filaments; IL: interlinks; LB: laminin binding; LETO: Long-Evans Tokushima Lean; MT: microtubules; N: nuclear; OLETF: Otsuka Long-Evans Tokushima Fatty; PM: plasma membrane; RI: Rho GDP-dissociation-inhibitor activity; ST: stress fibers; UN: unknown.