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. Author manuscript; available in PMC: 2011 Oct 10.
Published in final edited form as: Methods Enzymol. 2011;499:17–31. doi: 10.1016/B978-0-12-386471-0.00002-X

Figure 2.1.

Figure 2.1

Affinity chromatography of placental extract over Sepharose–α2M:Me. Fractions eluted from the α2M:Me–Sepharose affinity column were assessed by SDS-PAGE on a 5–15% gradient gel with a 4% stacking gel using the Laemmli buffer system. Lane 1, standards; lanes 2–5, fractions eluted from the affinity column. From Ashcom et al. (1990).