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. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Jul 21;255(3):251–260. doi: 10.1016/j.taap.2011.06.026

Figure 7. Concentration-dependent effect of TCDD on CD40L-induced expression of activation markers in human B cells.

Figure 7

Freshly isolated human B cells (from a different donor than presented in Figure 6) (1 × 106/ml) were treated with TCDD at concentrations indicated, and/or vehicle (VH), or remained untreated (NA) as a vehicle control and then co-cultured with irradiated CD40L-L cells (1.5 × 103 cell/well) in the presence of recombinant human IL-2 (10 U/ml), IL-6 (100 U/ml), and IL-10 (20 ng/ml) for 3 days. Cells were harvested right after isolation (Day 0, naive) and on Day 3, and then assessed by multiparametric flow cytometry for surface expression of CD80, CD86, and CD69. Dead cells were identified by staining with Live/Dead near-infrared Staining Kit, and are not included in these graphs. Data presented are the mean fluorescent intensity that represents the average expression level of each surface marker. *, p < 0.05, **, p < 0.01, compared to the VH-treated group. Three experimental replicates were included per group.

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