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. 2011 Oct 10;5:114. doi: 10.3389/fnins.2011.00114

Figure 4.

Figure 4

Immunocytochemical staining of rat pancreatic stem cells grown on cell culture plastic (left column), polyimide°1 from IMM (center column), and polyimide°2 from ISIT (right column). Cells are stained for alpha smooth muscle actin (SMA, first row), glial fibrillary acidic protein (GFAP, second row), nestin (third row), and neurofilaments (fourth row). Secondary antibody in green was FITC-labeled goat-anti-rabbit and in red was Cy3-labeled goat-anti-mouse. The nuclear counterstaining was made with DAPI. All scale bars represent 100 μm. Images are made with an Axioskop2 or Axio Observer.Z1 (both Carl Zeiss GmbH, Oberkochen, Germany).