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Immunocytochemical analysis of rat pancreatic stem cells grown on polyimide°2 film covered with fibrin. Cells are stained for alpha smooth muscle actin (SMA), glial fibrillary acidic protein (GFAP), nestin, and neurofilaments (NF). Secondary antibody in green was FITC-labeled goat-anti-rabbit and in red was Cy3-labeled goat-anti-mouse. The nuclear counterstaining was made with DAPI. Scale bars represent 100 μm (Axio Observer.Z1, Carl Zeiss GmbH, Oberkochen, Germany).
