Table 3.
OXPHOS Complex I and IV enzyme activities in mitochondria from hearts of 13- and 26-week-old female CD-1 mice exposed transplacentally to AZT, 3TC, or AZT-3TC
| Treatment group | Group sizes at 13 weeks (and 26 weeks)a | Complex activity ± SE at 13 weeks | Complex activity ± SE at 26 weeks | Comparisons of values at 13 versus 26 weeks of age (P-value) |
|---|---|---|---|---|
| Complex I enzyme activity b | ||||
| Control | n = 4 (3) | 49.3 ± 9.6 | 32.9 ± 19.1 | P > 0.05 |
| 3TC | n = 4 (4) | 67.0 ± 19.4 | 41.9 ± 4.0 | P > 0.05 |
| AZT | n = 4 (4) | 36.5 ± 12.5 | 29.5 ± 9.6 | P > 0.05 |
| AZT/3TC | n = 4 (4) | 41.1 ± 14.9 | 115.2 ± 44.9 | P > 0.05 |
| Comparisons of NRTI treatment groups versus controls (P-values) | P > 0.05 for all NRTI group versus control comparisons | P > 0.05 for all NRTI group versus control comparisons | ||
| Comparisons of individual NRTI treatment groups (P-values) | P > 0.05 for all NRTI group comparisons | P > 0.05 for all NRTI group comparisons | ||
| Complex IV enzyme activity b | ||||
| Control | n = 4 (3) | 109.5 ± 8.9 | 64.7 ± 25.0 | P > 0.05 |
| 3TC | n = 4 (4) | 146.0 ± 11.3 | 128.9 ± 15.3 | P > 0.05 |
| AZT | n = 4 (4) | 104.6 ± 29.7 | 98.9 ± 34.1 | P > 0.05 |
| AZT/3TC | n = 4 (4) | 139.8 ± 42.7 | 156.0 ± 38.4 | P > 0.05 |
| Comparisons of NRTI treatment groups versus controls (P-values) | P > 0.05 for all NRTI group versus control comparisons | P > 0.05 for all NRTI group versus control comparisons | ||
| Comparisons of individual NRTI treatment groups (P-values) | P > 0.05 for all NRTI group comparisons | P > 0.05 for all NRTI group comparisons |
a
Twenty-six-week groups sizes are given in parentheses
b
Enzyme activity values (μmol/min/mg protein ± SE) represent averages across the number of animals indicated ± standard error. Statistically significant differences between exposure groups and time points were not found using two-way ANOVA