. Author manuscript; available in PMC: 2011 Oct 10.

Published in final edited form as: Cardiovasc Toxicol. 2010 Jun;10(2):87–99. doi: 10.1007/s12012-010-9065-z

Table 4.

Sequence variants identified by PCR-based DGGE and DNA sequence analysis of mtDNA from hearts of control and NRTI-exposed female CD-1 mice

Exposure group, mouse number^a	Age (weeks)	Primer pair number	Location (base #)	Sequence variant
Unexposed controls (n = 3/19, 16%)
D108	13	11	9,821	–T base deletion
D126	26	11	9,821	+T base insertion
D130	26	11	9,821	+T base insertion
AZT-exposed^b (n = 2/15, 13%)
E002	26	11	9,821	+T base insertion
E023	26	4	(1) 3,815	–T base deletion
		11	(2) 9,821	+T base insertion
AZT/3TC-exposed^b (n = 3/19, 16%)
F104	13	11	9,821	–T base deletion
F105	26	11	9,821	–T base deletion
F110	26	4	3,815	–T base deletion

Number of litters examined: controls = 6 of 6 l, AZT = 5 of 6 l, 3TC = 3 of 3 l, AZT/3TC = 5of5 l

All exposures occurred during days 12–18 of gestation, and dosing was based on total body weight of the pregnant dam: 80 mg AZT/kg bw, 40 mg 3TC/kg bw, or 80 mg AZT + 40 mg 3TC/kg bw