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. 2008 Oct;17(5):869–881. doi: 10.1089/scd.2007.0240

FIG. 2.

FIG. 2.

HIFA and SSEA-4 (A–F) and HIF2A and OCT4 (G–L) expression in human embryonic stem cells (hESCs) cultured under either 20% (A–C and G–I) or 4% (D–F and J–L) O2. H1 cells (p33) were cultured for 10 days under either 20% or 4% O2 conditions. Immunofluorescent detection of HIF1A (red) and SSEA-4 (green) expression in colonies cultured under 20% (A and B) or 4% (D and E) O2. Spontaneous differentiation of H1 cells (d) under 20% O2 can be seen in the upper right (A–C) or bottom right corner (G–I) of the figure. Expression of HIF2A (green) and OCT4 (red) as observed by immunofluorescence in hESC colonies under 20% (G and H) or 4% (J and K) O2. Nuclei are stained blue using TO-PRO-3 (C, F, I, and L). All images were captured by confocal microscopy using a 20× objective. Bar, 0.1 mm.