Table 1.
List of Top 25 Genes Up and Down-Regulated in 20% O2
| Probe Set ID | Accession | Unigene | Symbol | Descriptions | Fold |
|---|---|---|---|---|---|
| Up-regulated in 20% O2 | |||||
| 209160_at | NM_003739 | Hs.78183 | AKR1C3 | Aldo-keto reductase family 1, member C3 | 2.2 |
| 225809_at | AI659927 | Hs.105460 | — | DKFZP564O0823 protein | 2.1 |
| 207057_at | NM_004731 | Hs.439643 | SLC16A7 | Solute carrier family 16 (monocarboxylic acid transporters), member 7 | 2.0 |
| 226278_at | AI150224 | Hs.349096 | — | Hypothetical protein DKFZp313A2432 | 2.0 |
| 226751_at | AW193693 | Hs.212885 | C2orf32 | Chromosome 2 open reading frame 32 | 1.8 |
| 230960_at | AI740721 | Hs.128292 | — | Transcribed locus | 1.7 |
| 211075_s_at | Z25521 | Hs.446414 | CD47 | CD47 antigen (Rh-related antigen, integrin-associated signal transducer)* | 1.7 |
| 235371_at | AI452595 | Hs.431092 | PPP4R2 | Protein phosphatase 4, regulatory subunit 2 | 1.7 |
| 230130_at | AI692523 | Hs.29802 | — | Transcribed locus | 1.7 |
| 209459_s_at | AF237813 | Hs.336768 | ABAT | 4-aminobutyrate aminotransferase | 1.6 |
| 205328_at | NM_006984 | Hs.26126 | CLDN10 | Claudin 10 | 1.6 |
| 201467_s_at | NM_000903 | Hs.406515 | NQO1 | NAD(P)H dehydrogenase, quinone 1* | 1.6 |
| 204493_at | NM_001196 | Hs.172894 | BID | BH3 interacting domain death agonist | 1.6 |
| 48808_at | NM_000791 | Hs.83765 | DHFR | Dihydrofolate reductase* | 1.6 |
| 223122_s_at | AF311912 | Hs.481022 | SFRP2 | Secreted frizzled-related protein 2 | 1.5 |
| 211737_x_at | BC005916 | Hs.371249 | PTN | Pleiotrophin (heparin binding growth factor 8)* | 1.5 |
| 225688_s_at | AK025444 | Hs.477114 | PHLDB2 | Pleckstrin homology-like domain, family B, member 2 | 1.5 |
| 209773_s_at | BC001886 | Hs.75319 | RRM2 | Ribonucleotide reductase M2 polypeptide | 1.4 |
| 232037_at | AW204060 | Hs.567396 | PUNC | Putative neuronal cell adhesion molecule | 1.4 |
| 225418_at | AI520949 | Hs.8372 | UQCR | Ubiquinol-cytochrome c reductase (6.4 kD) subunit | 1.4 |
| 1568678_s_at | BC037785 | Hs.487175 | FGFR1OP | FGFR1 oncogene partner | 1.4 |
| 201117_s_at | NM_001873 | Hs.75360 | CPE | Carboxypeptidase E | 1.3 |
| 215646_s_at | R94644 | Hs.443681 | CSPG2 | Chondroitin sulfate proteoglycan 2 (versican)* | 1.3 |
| 201202_at | NM_002592 | Hs.147433 | PCNA | Proliferating cell nuclear antigen | 1.3 |
| 202345_s_at | NM_001444 | Hs.408061 | FABP5 | Fatty acid binding protein 5 (psoriasis-associated) | 1.3 |
| Down-regulated in 20% O2 | |||||
| 1569287_at | BC017942 | Hs.621322 | — | Similar to otoconin 90* | −6.7 |
| 201848_s_at | NM_004052 | Hs.144873 | BNIP3 | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 | −4.6 |
| 201010_s_at | NM_006472 | Hs.533977 | TXNIP | Thioredoxin interacting protein* | −4.1 |
| 236180_at | W57613 | Hs.419240 | SLC2A3 | Solute carrier family 2 (facilitated glucose transporter), member 3 | −3.2 |
| 202718_at | NM_000597 | Hs.162 | IGFBP2 | Insulin-like growth factor binding protein 2 | −3.1 |
| 202887_s_at | NM_019058 | Hs.523012 | DDIT4 | DNA-damage-inducible transcript 4 | −3.0 |
| 1569453_a_at | BG772667 | Hs.560022 | — | Hypothetical locus LOC692247 | −2.9 |
| 206701_x_at | NM_003991 | Hs.82002 | EDNRB | Endothelin receptor type B* | −2.8 |
| 225283_at | AV70117 | Hs.6093 | ARRDC4 | Arrestin domain containing 4 | −2.6 |
| 1557636_a_at | BC031107 | Hs.258357 | — | Hypothetical protein LOC136288 | −2.5 |
| 1562529_s_at | BC040965 | Hs.569497 | RORA | RAR-related orphan receptor A | −2.4 |
| 206012_at | NM_003240 | Hs.25195 | LEFTY2 | Left-right determination factor 2 | −2.3 |
| 223734_at | AF329088 | Hs.84549 | OSAP | Corneal endothelium specific protein 1 | −2.2 |
| 207543_s_at | NM_000917 | Hs.500047 | P4HA1 | Procollagen-proline, 2-oxoglutarate 4-dioxygenase, alpha polypeptide I | −2.2 |
| 218717_s_at | NM_018192 | Hs.374191 | LEPREL1 | Leprecan-like 1 | −2.2 |
| 214624_at | NM_007000 | Hs.150309 | UPK1A | Uroplakin 1A | −2.2 |
| 223710_at | NM_006072 | Hs.131342 | CCL26 | Chemokine (C-C motif) ligand 26 | −2.1 |
| 201968_s_at | NM_002633 | Hs.1869 | PGM1 | Phosphoglucomutase 1* | −2.1 |
| 201105_at | NM_002305 | Hs.227751 | LGALS1 | Lectin, galactoside-binding, soluble, 1 (galectin 1) | −2.1 |
| 200737_at | NM_000291 | Hs.7877 | PGK1 | Phosphoglycerate kinase 1* | −2.0 |
| 227271_at | AU151265 | Hs.380704 | FGF11 | Fibroblast growth factor 11 | −2.0 |
| 206424_at | NM_000783 | Hs.150595 | CYP26A1 | Cytochrome P450, subfamily XXVIA, polypeptide 1 | −2.0 |
| 202022_at | NM_005165 | Hs.155247 | ALDOC | Aldolase C, fructose-bisphosphate | −2.0 |
| 227404_s_at | AI459194 | Hs.326035 | EGR1 | Early growth response 1 | −2.0 |
| 214823_at | AF033199 | Hs.8198 | ZNF204 | Zinc finger protein 204 | −1.9 |
Genes exhibiting an increase (Up-regulated) or decrease (Down-regulated) in all three samples (H1p37, H1p50, and H9p32) are represented. Fold changes shown are the average of the three paired comparisons. Positive or negative values indicate fold-increases or fold-decreases in gene expression, respectively, in human embryonic stem cells cultured under 20% O2 compared to cells under 4% O2. The complete list of genes which are consistently up- or down-regulated in 20% O2 is provided in Supplemental Table 3.
*
Genes represented by more than one probe-set, (redundant unigene entries were removed from list).