FIG. 5.

Analysis of conditioned media and the effects of secreted factors on cardiomyocyte hypertrophy in vitro. A, Quantitative ELISA of TGFβ1 and IGF1 concentration in progenitor cell-derived conditioned media. B, Representative images of cardiomyocytes stained with actin (green) for various control and experimental groups. C, Histogram showing increase in cell surface area for cardiomyocytes exposed to conditioned media (CM) from porcine and human endothelial progenitor cell cultures versus negative and positive controls, and recapitulation of pro-hypertrophic effect by addition of recombinant TGFβ1 to fresh media. There was no such effect mediated by IGF1 treatment. D, Attenuation of the pro-hypertrophic effect of human and porcine CM by neutralizing antibody to TGFβ1 but not by neutralizing antibody to IGF1. *indicates p < 0.05 for experimental versus negative control comparisons.